Use of a Redox Indicator to show Dehydrogenase Activity
Triphenyl tetrazolium chloride (also known as T.T.C) is an example of an artificial hydrogen acceptor. It is a redox indicator which is colourless when oxidised, however when reduced, it produces a red, insoluble precipitate called ‘formazans’. T.T.C can therefore be used to investigate the enzyme activity of dehyrogenase enzymes by showing a colour change when they are present. The purpose of this experiment is to […]