Please limit your answer to a maximum of 1 single-spaced, 11pt font, 1 inch margin, page.
In the paper on mature BDNF vs proBDNF in synapse competition by Je et al., PNAS, 109:15924-9. The authors suggest that mature BDNF promotes synapse stabilization.
1) Suggest one general mechanism to explain how BDNF might stabilize a synapse. In your explanation, state one specific hypothesis that you could experimentally test.
2) Describe one experiment to test your hypothesis.
3) You decide to perform a similar experiment with mammalian cells, using rat neurons and muscle cells. Similar to the Xenopus results (Fig.1 of Je et al.), you find that the activated synapse is stablilized, but the inactive one withdraws. Moreover, you find that adding a pan-matrix metalloprotease (MMP) inhibitor induces the retraction of both axons, even the active one (similar to Fig. 4 of Je et al.). You also find that there is no retraction of the inactive axon when using neurons from p75-/- mice. However, unlike the Je et al. results, you cannot detect any proBDNF released, and when you use an antibody that blocks binding of proBDNF to p75, there is no effect (the active synapse remains and the inactive one withdraws). Provide a possible explanation for these findings.
Delivering a high-quality product at a reasonable price is not enough anymore.
That’s why we have developed 5 beneficial guarantees that will make your experience with our service enjoyable, easy, and safe.
You have to be 100% sure of the quality of your product to give a money-back guarantee. This describes us perfectly. Make sure that this guarantee is totally transparent.
Read moreEach paper is composed from scratch, according to your instructions. It is then checked by our plagiarism-detection software. There is no gap where plagiarism could squeeze in.
Read moreThanks to our free revisions, there is no way for you to be unsatisfied. We will work on your paper until you are completely happy with the result.
Read moreYour email is safe, as we store it according to international data protection rules. Your bank details are secure, as we use only reliable payment systems.
Read moreBy sending us your money, you buy the service we provide. Check out our terms and conditions if you prefer business talks to be laid out in official language.
Read more